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医学论文范文:大黄素对模拟冷缺血再灌注后肝细胞内钙离子浓度及细胞凋亡的影响
【摘要】 目的 研究中药大黄素对模拟冷缺血再灌注后肝细胞内钙离子浓度及细胞凋亡的影响。方法 体外培养肝细胞株HL7702，随机分为对照组和大黄素处理组。对照组未予大黄素处理，大黄素处理组按100、10、1μmol/L分为高、中、低3个浓度组，建立模拟冷缺血再灌注模型，流式细胞技术检测各组细胞内钙离子浓度及细胞凋亡水平，分别检测各组细胞培养上清液乳酸脱氢酶水平。结果 冷缺血8h再灌注6h后，高、中、低浓度大黄素处理组钙离子荧光强度分别为(24.12±0.51)、(26.35±1.34)、(39.12±1.94)，均显著低于对照组的105.29±1.01(P<0.01)。高、中、低浓度大黄素处理组细胞凋亡率分别为(5.46±0.41)%、(10.64±0.64)%、(11.90±0.50)%，均显著低于对照组的(25.40±1.41)%(P<0.01)。高、中浓度大黄素处理组上清液LDH含量分别为(179.67±18.57)u/L、(198.83±14.22)u/L，显著低于对照组的(351.33±34.16)u/L(P<0.01)。结论 大黄素可降低模拟冷缺血再灌注后的肝细胞内钙离子浓度，抑制细胞凋亡，减轻肝细胞损伤。
【关键词】 大黄素;肝细胞;冷缺血再灌注;细胞内钙离子浓度;凋亡
Effect of Emodin on intracellular calcium concentration ([Ca2+]i) andapoptosis of hepatic cells after simulated cold ischemiareperfusion
QI Xiang, L Yi, SHEN Naiying, LIU Chang, LIU Xuemin, WANG Bo
Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical School ofXian Jiaotong University, Xian 710061, China
ABSTRACT: Objective To investigate the effect of Emodin on intracellular calcium concentration ([Ca2+]i) and apoptosis of hepatic cells after simulated cold ischemiareperfusion. Methods Glucoseoxygen deprivation, low temperature, subsequent reoxygenation and rewarming were used to induce ischemiareperfusion injury model in cultured hepatic cells which were divided into 4 groups: control group and Emodintreated group(100, 10 and 1μmol/L groups). The intracellular calcium concentration ([Ca2+]i) and apoptosis rate were determined by flow cytometry (FCM) respectively; the content of lactate dehydrogenase (LDH) in supernatant was tested. Results Intracellular calcium fluorescence intensity in Emodintreated groups of high, medium and low density was 24.12±0.51, 26.35±1.34 and 39.12±1.94, respectively, which were significantly lower than 105.29±1.01 in control group(P<0.01). Apoptosis rate in Emodintreated groups of high, medium and low density was 0.0546±0.0041, 0.1064±0.0064 and 0.1190±0.0050, respectively, which were significantly lower than 0.2540±0.0141 in control group(P<0.01). LDH was (198.83±14.22)u/L and (179.67±18.57)u/L in Emodintreated groups of medium and high density respectively, which were significantly lower than (351.33±34.16)u/L in control group(P<0.01). Conclusion Emodin could reduce [Ca2+]i and inhibit apoptosis of hepatic cells after simulated cold ischemiareperfusion, thus protecting hepatic cells effectively医.学全.在.线网站www.med126.com.
KEY WORDS: Emodin; hepatic cell; ischemiareperfusion injury; intracellular free calcium concentration; apoptosis
大黄素[12]化学结构为1，3，8羟基6甲基蒽醌，具有抗炎、抗肿瘤、保护肝肾等多种生物学作用。有文献报道[34]，大黄素可对细胞内钙离子浓度及细胞凋亡产生影响。钙超载及凋亡均为缺血再灌注损伤机制之一。本课题组前期研究发现[5]，大黄素对肝细胞体外模拟冷缺血再灌注损伤具有保护作用。该保护作用是否与抑制肝细胞内钙超载及凋亡有关?大黄素对冷缺血再灌注后肝细胞内钙离子浓度及凋亡的影响未见相关报道。本研究拟通过观察大黄素对模拟冷缺血再灌注后肝细胞内钙离子浓度及凋亡的影响，初步探讨其对冷缺血再灌注损伤可能的保护机制，为其在临床肝移植中的应用提供一定的实验依据。
